- Nathan Fillion
- Capt. Malcolm "Mal" Reynolds
- Gina Torres
- Zoë Warren
- Alan Tudyk
- Hoban "Wash" Washburn
- Morena Baccarin
- Inara Serra
- Adam Baldwin
- Jayne Cobb
- Jewel Staite
- Kaywinnit Lee"Kayle" Frye
- Sean Maher
- Dr. Simon Tam
- Summer Glau
- River Tam
- Ron Glass
- Shepard Meria Book
- Chiwetel Ejiofor
- The Operative
- David Krumholtz
- Mr. Universe
- Michael Hitchcock
- Dr. Mathias
- Sarah Paulson
- Dr. Caron
- Peter James Smith
- Lab Technician
- Joss Whedon
- Director, Writer
- Barry Mendel
- Producer
- Christopher Buchanan
- Executive Producer
- Alisa Tager
- Executive Producer
- Jack N. Green
- Cinematographer
- Barry Chusid
- Production Design
- Anya Colloff
- Casting
- Amy McIntyre Britt
- Casting
- Mark Brooks
- Original Music
- Carter Burwell
- Original Music
- Lisa Lassek
- Film Editing
