sprague-dawley rat中文, sprague-dawley rat中文意思
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sprague-dawley rat中文

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用"sprague-dawley rat"造句"sprague-dawley rat" in a sentence"sprague-dawley rat"怎麼讀

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  • 斯普拉-道來大鼠

例句與用法

  • Experiments were performed on adult male sprague - dawley rats weighing 230 - 270g
    本實驗采用健康成年雄性sd大鼠，體重2509士209 。
  • Enkephalin ( enk ) is one of endogenous opiate like substance and could induce psychological euphoria and modulate function of learning and memory . however there has no report available about the role of enk in acquisition of sprague - dawley rats as materials
    腦啡肽( enk )是內源性阿片樣物質的一種，可參與情緒反應調節，引起精神欣快感，并對學習記憶具有調制作用。
  • Methods : male sprague - dawley rats were decapitated . the thoracic aorta was removed and washed in cold krebs - henseleit solution , then cut into 2 - 3 mm in length . the endothelium of some rings was removed by rubbing with a metal thread
    取雄性sprague - dawley大鼠的胸主動脈條上段，置于4的k - h液中，將血管剪成2 3mm長的主動脈環，部分血管環先用機械法去除血管內皮，迅速懸掛至離體灌流裝置中。
  • In this experiment , radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process . methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0 . 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al . thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum , and added to 50ml cell culture flasks
    方法：胰島的分離參照王玲等的方法，每次實驗取新生3 - 5天sd大鼠，無菌條件下剖腹取出胰腺，剪切為0 . 5mm ~ 3的組織塊， v型膠原酶消化30min后，離心洗滌，懸浮于完全培養基，接種入50ml培養瓶，于5 co _ 2 、 95空氣條件下培養20h ，轉板純化，接種于96孔培養板培養24h ，按實驗要求進行實驗。
  • Primary culture of rat preadipocyte were prepared from the epididymal , inguinal and perirenal the fat pads of male normal , healthy , 15 - 20 days sprague - dawley rats . the preadipocyte grew better under the condition of 37 , 95 % humidity , 5 % co2 , ph 7 . 0 - 7 . 2 , centrifuged at 1000r / min , m199medium , and 10 % fetal bo vine serum , seeded at a density of 4 l04 , 5 l04 , / cm2 . oil red o staining was the special method to distinguish adipocyte from other cells , gimsa and he could determine the stage of the adiopcyte differentiation through the number of lipid drop , size and the position of the nucleolus of the staining fat cell
    經過多次實驗，確定本實驗室大鼠前體脂肪細胞的最佳培養條件是：溫度為37 ，濕度為95 ， co _ 2濃度為5 ， ph值為7 . 0 7 . 2 ，離心力為1000r / min ，培養基為m _ ( 199 )培養基，胎牛血清濃度為10 ，合適細胞接種密度為4 10 ~ 4 、 5 10 ~ 4個/ cm ~ 2 ，染色結果表明：油紅o染色是鑒定脂肪細胞的特異方法， gimsa和he染色可根據不同區域染色程度、著色差別判斷細胞核的位置及脂滴大小、多少，觀察大鼠前體脂肪細胞分化過程中的形態變化，進而確定脂肪細胞的分化階段。
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