- Tobey Maguire
- Peter Parker/Spider-Man
- Willem Dafoe
- Norman Osborne/Green Goblin
- Kirsten Dunst
- Mary Jane Watson
- James Franco
- Harry Osborn
- Cliff Robertson
- Ben Parker
- Rosemary Harris
- May Parker
- J.K. Simmons
- J. Jonah Jameson
- Joe Manganiello
- Flash Thompson
- Gerry Becker
- Maximillian Fargas
- Bill Nunn
- Joseph "Robbie" Robertson
- Jack Betts
- Henry Balkan
- Stanley Anderson
- General Slocum
- Ron Perkins
- Dr. Mendel Stromm
- Sam Raimi
- Director
- David Koepp
- Writer
- Stan Lee
- Executive Producer, Writer
- Steve Ditko
- Writer
- Avi Arad
- Executive Producer
- Ian Bryce
- Producer
- Laura Ziskin
- Producer
- Grant Curtis
- Co-Producer
- Don Burgess
- Cinematographer
- Neil Spisak
- Production Design
- Bob Murawski
- Film Editing
- Arthur Coburn
- Film Editing
- John Dykstra
- Visual Effects Designer
- James Acheson
- Costume Design
- Danny Elfman
- Original Music
- Francine Maisler
- Casting
- Lynn Kressel
- Casting
