Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and LeavesRegina Schweiger 1, Serena Schwenkert 1
1Department Biologie I, Botanik, Ludwig-Maximilians-Universität, München
Formation of protein complexes in vivo can be visualized by bimolecular fluorescence complementation. Interaction partners are fused to complementary parts of fluorescent tags and transiently expressed in tobacco leaves, resulting in a reconstituted fluorescent signal upon close proximity of the two proteins.
