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Entamoeba chiangraiensis n. sp. (Amoebozoa: Entamoebidae) isolated from the gut of Asian swamp eel (Monopterus albus) in northern Thailand

Published online by Cambridge University Press:  23 October 2019

Vasana Jinatham ,
Siam Popluechai ,
C. Graham Clark  and
Eleni Gentekaki Open the ORCID record for Eleni Gentekaki [Opens in a new window]
Vasana Jinatham
Affiliation:
School of Science, Mae Fah Luang University, Chiang Rai, 57100Thailand
Siam Popluechai
Affiliation:
School of Science, Mae Fah Luang University, Chiang Rai, 57100Thailand Gut Microbiome Research Group, Mae Fah Luang University, Chiang Rai, 57100Thailand
C. Graham Clark
Affiliation:
Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK
Eleni Gentekaki*
Affiliation:
School of Science, Mae Fah Luang University, Chiang Rai, 57100Thailand Gut Microbiome Research Group, Mae Fah Luang University, Chiang Rai, 57100Thailand
*
Author for correspondence: Eleni Gentekaki, E-mail: gentekaki.ele@mfu.ac.th
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Abstract

The genus Entamoeba comprises mostly gut parasites and commensals of invertebrate and vertebrate animals including humans. Herein, we report a new species of Entamoeba isolated from the gut of Asian swamp eels (Monopterus albus) in northern Thailand. Morphologically, the trophozoite is elongated and has a single prominent pseudopodium with no clear uroid. The trophozoite is actively motile, 30–50 µm in length and 9–13 µm in width. Observed cysts were uninucleate, ranging in size from 10 to 17.5 µm in diameter. Chromatin forms a fine, even lining along the inner nuclear membrane. Fine radial spokes join the karyosome to peripheral chromatin. Size, host and nucleus morphology set our organism apart from other members of the genus reported from fish. The SSU rRNA gene sequences of the new isolates are the first molecular data of an Entamoeba species from fish. Phylogenetic analysis places the new organism as sister to Entamoeba invadens. Based on the distinct morphology and SSU rRNA gene sequence we describe it as a new species, Entamoeba chiangraiensis.

Keywords

Archamoebaeintestinal protistmorphologyphylogenySSU rRNA
Type
Research Article
Information
Parasitology , Volume 146 , Special Issue 14: BSP 2018 Autumn Symposium , December 2019 , pp. 1719 - 1724
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Copyright
Copyright © Cambridge University Press 2019 

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Footnotes

ZooBank ID: urn:lsid:zoobank.org:act:1BAAFBDF-137F-4046-A196-76C2B5D1D70A

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