MT 26 NON-PATHOGENIC INTESTINAL AMEBAE

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Terms in this set (41)

Entamoeba dispar
Entamoeba coli
Entamoeba hartmanni
Entamoeba gingivalis
Iodamoeba butschlii
Endolimax nana
Blastocystis hominis
Entamoeba polecki

non-pathogenic

cytoplasm: fine granular (ground-glass appearance)

inclusions: bacteria nucleus: single with evenly distributed
peripheral chromatin (beadlike arrangement)

karyosome: small, centrally located
movement: progressive & purposeful or directional

E. dispar

cytoplasm
inclusions
nucleus
karyosome
movement

Entamoeba dispar

morphologically identical with E. histolytica but genetically different (DNA and ribosomal RNA)

non-invasive or non-pathogenic

Inclusions: never contain RBCs

: bacteria or debris in vacuoles

E. dispar trophozoite inclusions

◼ If trophozoites present do not have ingested red blood cells on their cytoplasm.

◼ If cysts stage is present.

When to report results as E. histolytica/E. dispar by microscopy:

Entamoeba hartmann

known as the small-raced E. histolytica

Cytoplasm: finely granular

Inclusions: may contain bacteria ; no RBCs

Nucleus: usually one (difficult to see in unstained preparation);

Peripheral chromatin: may appear as solid ring rather than beaded

Karyosome: usually small and compact; may be centrally located or eccentric

Motility: usually non-progressive; less vigorous than E. histolytica

Entamoeba hartmanni trophozoite

cytoplasm
inclusions
nuckeuss
peripheral chromatin
karyosome
motility

Nucleus: mature cyst - 4 ; immature - two nucleated cysts very common

chromatoidal bodies: short with tapered ends or thin and bar-like;

Entamoeba hartmanni cyst

nuclues
chromatoidal bodies

Entamoeba coli

◼ often coexist with E. histolytica
◼ more common that E. histolytica due its superior ability to survive in putrefaction

cytoplasm - coarsely granular (dirty appearance); usually vacuolated;

inclusions: bacteria, yeast, other debris

peripheral chromatin - may be clumped and unevenly distributed appearing as solid, dark ring

karyosome - large, not compact; usually eccentric

motility - sluggish, non-directional with blunt, granular pseudopodia

Entamoeba coli trohpozoite

cytoplasm
inclusions
perpheral chromatin
karyosome
motility

shape - variable

nucleus - mature cyst - 8; occasionally 16 or more;

chromatoidal bodies - splinter shaped with rough, pointed ends (broom stick appearance)

Entamoeba coli cyst

shape
nucleus
chromatoidal bodies

Endolimax nana

live both in vertebrates and invertebrates (large intestine)

cytoplasm - granular, vacuolated

inclusions: may contain bacteria

peripheral chromatin - usually absent

karyosome - large, compact & irregularly shaped; may appear "blot-like; may be centrally located or eccentric

motility - sluggish, usually non-progressive

Endolimax nana trohpozoite

cytoplasm
inclusions
peripheral chromatin
karyosome
motility

nucleus - mature cyst - 4 ; may resemble cysts of Enteromonas hominis

chromatoidal bodies - rarely present; occasionally small granules or faintly visible fine linear bodies

Endolimax nana cyst

nucleus
chromatoidal bars

Iodamoeba butchlii

4-8%

◼ worldwide in distribution
◼ infects humans, pigs and other primates
◼ the most common ameba in swine (probably original host)
◼ prevalence in human - _