重新定義益生菌功效！新創益生菌 TW01 可降低大腸癌、抗PM2.5

人體90%疾病竟跟乳酸菌有關

人體消化道從口腔、食道、胃、小腸、大腸、直腸到肛門，總長度約6~9公尺，表面積約為280平方公尺，全部攤開有一個網球場大，且腸道佔據了人體70%免疫能力。

面臨自然界到處充滿看不見的威脅，人體每天都在開戰。面對病原菌的入侵，腸道中的乳酸菌將作為對抗的第一道防線。而事實上乳酸菌不只是侷限於腸道作用，縱觀從眼睛、私密處、泌尿道、上呼吸道，這些都與腸道乳酸菌有著千絲萬縷的關係。

偌大腸道中就有上百兆的共生菌、上千類的菌種，它們遍及人體各地，提供完整的佈防機制。由於菌叢生態數目非常大，菌種種類又多，故平時益生菌應以長期補充，且以菌種多樣化的方式進行。而益生菌在定植腸道後，會產生有益代謝物，便能啟動免疫防禦及降低腸內毒素，進而減少腸漏現象、降低毒素竄流器官，以達到理想的健康狀態。 

重新定義益生菌，台灣人的益生菌

市售益生菌那麼多，是否有適合亞洲人補充的菌株？又或者說，益生菌是否分成東西方體質？其實正確來講，所謂的東西方體質益生菌，指的是飲食及生活在不同情況下，腸道裡面的菌叢生態也會產生不同的變化。

例如：西方人油脂吃的多，腸道就發展出較多能分解油脂的「擬桿菌」；東方人愛吃米食，腸道就發展出較多能增加醣類代謝的「普氏菌」，尤其愛吃纖維的人，普氏菌更高達60％。所以這證明了，我們的飲食會偏愛某種乳酸菌的定植率。

只要借助這個邏輯，就可以逆向研究台灣國人的飲食，找出頻率最高的食物。再從這食物中找出相伴相隨的乳酸菌，提煉出來做為國人的益生菌，這樣就很符合台灣人的益生菌。

根據國際咖啡組織（ICO）統計，台灣平均每人每年喝掉122杯咖啡。在如此高頻率的飲食下，新創益生菌 TW01 （Lactobacillus acidophilus TW01）就是從此契機中誕生。 TW01 是從台灣古坑原生咖啡豆中進行發酵，且成功從發酵液的上千株菌中，找到唯一有效的菌株。耗時一年半的找尋，最終找到符合台灣人的飲食習慣，適合台灣人的菌株。

不單如此，乳酸菌的存活易受 pH 值影響，特別是在 pH3 以下會對菌體產生最大傷害。在相關研究中大多是以 pH3 進行耐酸測試。但新創益生菌「TW01」，是在更嚴苛的 pH2 條件下測試30分鐘，發現菌數竟無太大損傷，證實耐酸程度強悍。另耐鹼測試以0.3%膽鹽進行，也表現出耐鹼能力（如圖二）。

三效合一，新創益生菌TW01

台灣首支三效合一益生菌 Lactobacillus acidophilus TW01 證實可降低大腸癌風險、抗PM2.5、活化免疫平衡。TW01 是由江欣樺營養師領軍開發，正式攻破國人十大死因中的大腸癌、肺炎，帶領益生菌走向新紀元。 

TW01 益生菌功效一、降低大腸癌風險

新創益生菌 TW01，進入腸道吸取碳源當作養分，之後進入貝爾斑進行免疫調節，並促使B細胞分泌免疫球蛋白IgA，且IgA處在腸道黏膜最外層的黏液層，可以阻擋細菌對上皮細胞的破壞，減少腸漏現象，進而減少腸內毒素產生（如圖三）。

研究成果
經實驗證實，TW01菌數從百萬/CFU到1億/CFU，均發現能抑制 Caco2 （人類結腸腺癌細胞），也就是抑制大腸癌細胞，且隨菌數越高抑制效果越好。不僅如此，在 TW01 作用下，還能促進腸道細胞傷口復原。（如圖四）

TW01 益生菌功效二、抗PM2.5

1. TW01 可以強化腸道黏膜，降低內毒素進入肺部。並且 TW01 所產生的短鏈脂肪酸（SCFA）及細菌素，能抑制腸內壞菌滋生。而 SCFA 可做為腸黏膜上皮細胞能量來源，可以強化腸道黏膜的完整性，避免內毒素進入血液循環，並經肺—腸軸線，因此能降低毒素進入肺部機率。（如圖五）
2. TW01 降低呼吸道損傷，經免疫反應所產生的 IgA 成為了上呼吸道對抗細菌、病毒能力。（如圖五）
3. TW01 透過肺—腸軸線將抗氧化物送至肺部，可以降低肺部損傷。（如圖五）

研究成果
經實驗證實， TW01 能有效提升抗氧化物質，使呼吸道對抗 PM2.5 的能力提升一倍，且 TW01 也能大幅提升受損細胞的存活率。面對受到PM2.5 損害的呼吸道，TW01具有先防禦、後修復的趨勢能力。（如圖六）

TW01 益生菌功效三、達到免疫調節

人體內有非常多種生化因子，並且會不斷傳送訊號，決定是否要產生免疫攻擊，以及攻擊的程度有多強、攻擊的時間有多長。免疫反應太低，保護能力就會不足；免疫反應太強，身體就會受損，甚至引起過敏。（如圖七）

研究成果
從研究結果看來，TW01能夠喚起許多具有指標意義的免疫細胞激素，同時又能啟動緩解反應的細胞因子。對於免疫系統與相關疾病來說，TW01能夠啟動也能夠節制，讓免疫的戰力增強，但也更有紀律。（如圖八）

新世紀材料，TW01也能當作後生元

後生元是益生菌的代謝產物，具耐高溫、耐鹽鹼、耐胃酸特性。研究團隊也發現，當 TW01 成為死菌後，也變成了「後生元」，並且同樣具有免疫調節的能力。這項發現增加了 TW01 在食品加工的應用範圍，很適合當成補湯類的功能性添加，例如雞精或魚精。（如圖九）

1+1大於2，TW01啟動體內額外好菌繁殖

市售益生菌中，最大的兩群益生菌便是乳酸桿菌屬（Lactobacillus）及雙歧桿菌屬（Bifidobacterium）。眾所皆知腸道裡的菌都會搶地盤，當益生菌進入腸道後，約一周內就會定植下來，並分泌有益的代謝物（如短鏈脂肪酸、細菌素）。短鏈脂肪酸能穿透壞菌，抑制其能量代謝使壞菌餓死而減少，也會試圖增加好菌地盤的優勢，讓蠢蠢欲動的壞菌不敢造次。

此外，經實驗證實，TW01屬於乳酸桿菌屬（Lactobacillus）在發揮其功能時，竟也能提高不同屬間的雙歧桿菌增長。意思是TW01自己能打仗還能找到同盟國作戰，這項發現無疑對腸道好菌的地盤多了一份不可撼動的力量。（如圖十）

榮獲國家新創獎，台灣人的益生菌 

台灣首支三效合一益生菌（Lactobacillus acidophilus TW01），江欣樺營養師表示，因自己鼻竇炎且長期受空氣污染PM2.5困擾，也為了想找到適合台灣人的益生菌，一心投入學界開始研發。最終，在台灣古坑咖啡豆發酵液裡面的上千株菌中，找到唯一有效的菌株。

TW01益生菌耗時三年開發，研發經費已高達2千萬台幣，但取得成就非凡，並獲得2023年國家新創獎，獲得國家肯定，也是該年度益生菌品類中唯一獲獎者。並且 TW01 獲得台灣發明專利、美國發明專利、日本發明專利、法國艾菲爾國際發明競賽金獎及加拿大發明特別獎。希望此菌株TW01能造福更多台灣人的健康，揚名國際，為台灣爭光。

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50. Systematic Investigation of the Effect of Lactobacillusacidophilus TW01 on Potential Prevention of Particulate Matter(PM)2.5-Induced Damage Using a Novel In Vitro Platform Sioumin Luo and Mingju Chen *