- Tommy Lee Jones
- MiB Agent K (Kay)
- Will Smith
- Officer James Darrel Edwards III/MiB Agent J (Jay)
- Linda Fiorentino
- Deputy Medical Examiner Laurel Weaver, M.D./MiB Agent L (Elle)
- Vincent D'Onofrio
- Edgar/Edgar Bug
- Rip Torn
- MiB Chief Z (Zed)
- Tony Shalhoub
- Jeebs
- Sergio Calderon
- Jose
- Siobhan Fallon
- Beatrice
- Carel Struycken
- Arquillan
- Michael Willis
- Cop in Morgue
- Barry Sonnenfeld
- Director
- Lowell Cunningham
- Writer
- Ed Solomon
- Screenwriter
- Laurie MacDonald
- Producer
- Steven R. Molen
- Associate Producer
- Walter F. Parkes
- Producer
- Graham Place
- Co-Producer, Unit Production Manager
- Steven Spielberg
- Executive Producer
- Danny Elfman
- Original Music
- Donald Peterman
- Cinematographer
- Jim Miller
- Film Editing
- David Rubin
- Casting
- Debra Zane
- Casting
- Bo Welch
- Production Design
- Tom Duffield
- Art Director
- Cheryl Carasik
- Set Decoration
- Mary E. Vogt
- Costume Design
- Amy Ness
- Production Supervisor
