- Wesley Snipes
- Blade, Producer
- Kris Kristofferson
- Abraham Whistler
- Jessica Biel
- Abigail Whistler
- Ryan Reynolds
- Hannibal King
- Parker Posey
- Danica
- Dominic Purcell
- Dracula/Drake
- John Michael Higgins
- Dr. Edgar Vance
- James Remar
- Ray Cumberland
- Eric Bogosian
- Bentley Tittle
- Patton Oswalt
- Hedges
- Callum Keith Rennie
- Asher
- Natasha Lyonne
- Sommerfield
- Mark Berry
- Chief Martin Vreede
- Steve Braun
- FBI Agent Wilson Hale
- Triple H
- Jarko Grimwood
- David S. Goyer
- Director, Producer, Screenwriter
- Avi Arad
- Executive Producer, Producer
- Peter Frankfurt
- Producer
- Lynn Harris
- Producer
- Wesley Snipes
- Blade, Producer
- Cale Boyter
- Executive Producer
- Toby Emmerich
- Executive Producer
- Stan Lee
- Executive Producer
- Gabriel Beristain
- Cinematographer
- Howard E. Smith
- Film Editor
- Conrad Smart
- Film Editing
- Ramin Djawadi
- Original Music
- RZA
- Original Music
- Chris Gorak
- Production Design
- Erin Fraser
- Art Director
- Patrick Banister
- Art Director
- Eric Fraser
- Art Director
- Ted Kuchera
- Set Decoration, Set Designer
- Laura Jean Shannon
- Costume Design
- Ronnie Yeskel
- Casting
- Patrick Ramsay
- Sound
- George A. Grieve
- Unit Production Manager
- Pete Whyte
- First Assistant Director
- Vic Armstrong
- Second Unit Director
