Mighty Mouse Playhouse was an American television anthology series featuring animated short films starring Mighty Mouse. The series aired on CBS from 1955 to 1966. The series was credited with popularizing the Mighty Mouse character in popular culture far beyond what the original film shorts had done.

Mighty Mouse was not extraordinarily popular in theatrical cartoons, but was still Terrytoons' most popular character. What made him a cultural icon was television. Most of the short film studios, both live-action and animated, were in decline by the 1950s, pressured both by the loss of film audiences to television as well as the increased popularity of low-budget, stylized, limited animation. Most of the studios cashed out of the short-film production business and began licensing or selling their back catalogs to television. Paul Terry went as far as to sell the entire Terrytoon company to CBS in 1955. The network began running Mighty Mouse Playhouse in December 1955. It remained on the air for nearly twelve years. Mighty Mouse cartoons became a staple of children's television programming for a period of over thirty years, from the 1950s through the 1980s.